Reporter

Part:BBa_K215010

Designed by: Jeff Nivala   Group: iGEM09_Washington   (2009-10-01)

pLac+RBS+GFP with Secretion Signal and Streptavidin Binding Tags on GFP

This part is GFP (E0040) amplified out and inserted into K215002 at the NheI cut site then put into the expression vector K215000. For information about this parts functionality when expressed please see BBa_K215002 page or the [http://2009.igem.org/Team:Washington University of Washington 2009 project Wiki].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 842


[edit]
Categories
Parameters
n/apLac+RBS+GFP with Secretion Signal and Streptavidin Binding Tags on GFP